bamhi digested pcep4 plasmid Search Results


pcep4 expression vector  (Thermo Fisher)
90
Thermo Fisher pcep4 expression vector
Pcep4 Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the pcep4 cloning vector  (Thermo Fisher)
90
Thermo Fisher the pcep4 cloning vector
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
The Pcep4 Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the pcep4 cloning vector - by Bioz Stars, 2026-02
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pcep4 myc ace2  (Addgene inc)
93
Addgene inc pcep4 myc ace2
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Myc Ace2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4-mir302-367  (Addgene inc)
90
Addgene inc pcep4-mir302-367
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Mir302 367, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4-p53cd75  (Thermo Fisher)
90
Thermo Fisher pcep4-p53cd75
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 P53cd75, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression construct dna  (Thermo Fisher)
99
Thermo Fisher expression construct dna
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Expression Construct Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4 bcl 2  (Addgene inc)
93
Addgene inc pcep4 bcl 2
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Bcl 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4 m2l  (Addgene inc)
93
Addgene inc pcep4 m2l
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 M2l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcep4 m2l/product/Addgene inc
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pcep4 oct4 t234e s235e expression vectors  (Addgene inc)
92
Addgene inc pcep4 oct4 t234e s235e expression vectors
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Oct4 T234e S235e Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pecfp n1 vector  (Addgene inc)
91
Addgene inc pecfp n1 vector
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pecfp N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4 vector  (Thermo Fisher)
86
Thermo Fisher pcep4 vector
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4 vectors sp-gfpapp695  (Thermo Fisher)
90
Thermo Fisher pcep4 vectors sp-gfpapp695
Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the <t>pCEP4</t> cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).
Pcep4 Vectors Sp Gfpapp695, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the pCEP4 cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).

Journal:

Article Title: Tracking an embryonic L1 retrotransposition event

doi: 10.1073/pnas.0337627100

Figure Lengend Snippet: Retrotransposition of L1-EGFP. (a) Schematic of the L1-EGFP transgene and its retrotransposition. L1 transcription is driven by the mouse RNA pol II promoter in addition to the L1 5′ UTR (21). The EGFP gene is in the antisense orientation relative to L1. EGFP (green) is situated in the 3′ UTR (hatched) of L1 and is interrupted by the mouse γ-globin intron. The intron is in the same transcriptional orientation as L1 (18). Therefore, when the L1 sense-strand transcript is processed, the γ-globin intron is spliced out. The EGFP gene is driven by the human CMV MIE promoter (pCMV-MIE) and has an HSV thymidine kinase polyadenylation sequence (tkpA). pCMV-MIE, EGFP, and tkpA are all antisense relative to L1RP. At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the pCEP4 cloning vector (Invitrogen). 5′ truncation of the L1-EGFP insertion is depicted with a jagged line. Arrows depict the locations of the geno5 (left) and geno3 (right) genotyping primers used in the PCR assay shown in b (not drawn to scale). (b) The geno5 and geno3 primers flank the intron in EGFP and give rise to two products, a 1.5-kb amplicon (corresponding to the intron-containing transgene) and an ≈600-bp amplicon that lacks the 909-bp intron (corresponding to the insertion). Shown are the genotyping results on tail DNA from five offspring of founder 57 (lanes 1–5). dw, distilled water; neg, genomic DNA from the tail of a transgene negative mouse; tg, L1-EGFP transgene; XIV, 100-bp ladder with bright bands at 500 bp, 1,000 bp, and 2.6 kb (top band; Roche).

Article Snippet: At the very 3′ end of the L1-EGFP transgene is the SV40 late polyadenylation sequence (SV40pA) derived from the pCEP4 cloning vector (Invitrogen).

Techniques: Sequencing, Derivative Assay, Clone Assay, Plasmid Preparation, Amplification